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KMID : 0365819710110020109
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Journal of Pusan Medical College
1971 Volume.11 No. 2 p.109 ~ p.123
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A Comparative Histochemical Study on the Mucosa of the Gall Bladder and Common Bile Duct
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Abstract
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A comparative histochemical study was done on the histochemical nature of mucosubstances and distribution of alkaline phosphatase and acid phosphatase in the mucosa of the gall bladder and common bile duct, using the dog, cat, rabbit, guinea pig and human fetus. The mucosubstance was observed with alcian blue(pH 2.5), PAS, mucicannine and combined staining of alcian blue(pH2. 5) and PAS. The
alkaline and acid phosphatase were observed with f omori´s modified methods.
The results were as follows:
A.Histological findings.
1. The epithelium of the gall bladder in the dog, cat, rabbit and guinea pig consisted of two sorts of calls, one with tall slender columnar and strongly eosinophilic, the other with low wide columnar and weakly stainable with eosin, but the epithelium of the gall bladder in the human
fetus consisted of single columnar cells with elliptical nuclei which was faintly stainable. The goblet cells were not recognized in the epithelium of the gall bladder of the animal materials and human fetus.
2. The tall slender columnar cells were mainly scattered near the surface of fold, on the other hand, the low wide columnar cells were arranged in groups and mainly visible in the base and bending portion of fold in the all animal materials.
3. The epithelium of the common bile duct in the all animal materials and human fetus consisted of sima1e calum:32r calls and also soma goblet cells. Moreover, the intraepithelial mucous glands were recognized in the guinea pig and the goblet cells in the human fetus were larger and contained coarser mucous granules than in the animal materials.
4. The biliary glands in the dog and cat were simple tubular glands consisting of simple columnar calls and goblet cells which ware formed by the invagination of epithelium. But the biliary glands in the rabbit, guinea pig and human fetus were mostly mucous glands and sometimes mixed glands.
B. Histochemical findings.
1. The tall slender cells in the gall bladder epithelium were not stained with alcian blue but stained
weakly to moderately with PAS in the all animal materials, mucicarmine staining was weakly positive only in the dog, and combined staining of alcian blue and PAS was negative in the rabbit and guinea pig but weakly to moderately positive only to PAS in the dog and cat,
2. The low wide columnar cells in the gall bladder epithelium were stained weakly to moderately with alcian blue in the all animal materials except of the rabbit whose was not stainable, and PAS was strongly positive in the dog but weakly positive in the all other animal materials. Mucicarmine staining was negative in the rabbit and guinea pig but weakly to moderately positive in the cat and dog, and combined staining of alcian blue and PAS was negative in the rabbit and strongly positive in equal strength in the dog but more strongly to PAS in the cat and weakly only to alcian blue in the guinea pig.
3. The epithelium of the gall bladder in the human fetus was scarcely stainable with alcian blue, moderately positive with PAS, weakly positive with mucicarmine and moderately positive but stronger to alcian blue with combined staining of alcian blue and PAS.
4. The goblet cells of the common bile duct stained weakly in the dog, cat and rabbit and moderately in the guinea pig and human fetus with alcian bule. PAS was moderately to strongly positive in the all materials and mucicarmine staining was weakly positive except of the dog whose was moderately positive, and combined staining of alcian blue and PAS was moderately to strongly positive in equal strength in the cat and dog but more stronger to PAS in the rabbit, guinea pig and human fetus
5. The mucous glands in the lamina propria of the common bile duct stained moderately to strongly with alcian blue, weakly to moderately with PAS, weakly to moderately with mucicarmine and moderately to strongly but only or more to alcian blue with combined staining of alcian blue and PAS in the rabbit, guinea pig and human fetus.
6. The reaction of alkaline phosphatase in the gall bladder mucosa was strongly positive in the surface epithelium and moderately positive in the base epithelium in the dog but strongly positive only in the surface epithelium in other animal materials and scarcely positive in the free border of surface epithelium in the human fetus. The reaction of acid phsophatase was moderately positive in the surface and base epithelium in the dog but weakly positive only in the surface epithelium in other animal materials and negative in the human fetus.
7. The reaction of alkaline phosphatase in the surface epithelium of the common bile duct was moderately positive in the dog, weakly positive in the guinea pig but negative in the cat, rabbit and human fetus. The reaction of acid phosphatase was weakly to moderately positive in the surface epithelium of the dog but negative in the all other materials including human fetus.
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